{"created":"2023-05-15T14:32:53.042526+00:00","id":290,"links":{},"metadata":{"_buckets":{"deposit":"57097e21-a6da-4dbd-be4b-13e86e9870a3"},"_deposit":{"created_by":4,"id":"290","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"290"},"status":"published"},"_oai":{"id":"oai:uekusa.repo.nii.ac.jp:00000290","sets":["1","1:63"]},"author_link":["490","491","492","493","494"],"control_number":"290","item_3_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03-01","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"159","bibliographicPageEnd":"119","bibliographicPageStart":"113","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"植草学園大学研究紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of Education and Health Sciences, Uekusa-Gakuen University","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"三叉神経脊髄路尾側亜核は顔面の三叉神経支配領域に出現する疼痛の中継核だけでなく，偏頭痛の発生源とも考えられている。核内にはGABAなど抑制性伝達物質をもつニューロンが多数存在し，痛覚求心性神経情報の２次処理を行っており，その神経回路網構築と処理様式の解析は，慢性疼痛の予防・治療法を開発する上で必須である。本研究では，GAD67（GABA合成酵素・グルタミン酸デカルボキシラーゼ）のかわりに，発色同定の目的でgreen fluorescence protein（GFP）遺伝子を組み込んだ新生マウス（GAD67-GFPノックインマウス）の三叉神経付脳幹スライス標本を作成し，三叉神経根の電気刺激に対する尾側亜核内のGABA性および非GABA性二ューロンの応答をホールセルパッチクランプ法により解析した。GABA性ニューロンは，20例中17例で興奮性入力のみを，３例で興奮性と抑制性の両入力を受けているものがあった。これに対し，非GABA性ニューロンは，興奮性入力を受けているものが31例中18例，興奮性入力と抑制性入力の両方を受けているものが11例，抑制性入力のみのもの２例であった。以上の結果から，尾側亜核内のGABA性ニューロンは求心神経より興奮性入力を受け，非GABA性ニューロンの興奮を抑制していることが推定できる。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_3_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The neurons of the trigeminal spinal tract subnucleus caudalis (Sp5C) play an important role in the processing of pain and sensory information from the cranio-facial area. In the present study, we investigated the connections of GABAergic, non-GABAergic neurons, and the primary sensory afferents (C-fibers) in the Sp5C, using neonatal transgenic (GAD67-GFP knock-in) mice in which green fluorescent protein (GFP) is specifically expressed in GABAergic neurons. We recorded activities of GFP-positive (GABAergic) and GFP-negative (non-GABAergic) neurons using the whole-cell patch clamp technique in the sagittal slice of brainstem attaching the trigeminal nerve rootlet. Electrical stimulation of the trigeminal nerve rootlet evoked excitatory postsynaptic currents (EPSCs) in 20 GFP-positive and in 31 GFP-negative neurons. Inhibitory postsynaptic currents (IPSCs) were evoked in 3GFP-positive and in 13 GFP-negative neurons. In morphological study, most GFP-positive neurons had multipolar-shaped somata (n=8/10) and GFP-negative neurons had bipolar (n=5/10) or triangular (n=5/10) somata. The size of each neuron is not significantly different. Therefore, we conclude that GABAergic neurons in the Sp5C receive exclusively excitatory postsynaptic inputs, and inhibit the activities of non-GABAergic neurons in the same nucleus.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"14","subitem_description_type":"Other"}]},"item_3_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00008523560","subitem_description_type":"Other"}]},"item_3_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA12412714","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18835988","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAlternatives":[{"creatorAlternative":"KUWANA, Shunichi","creatorAlternativeLang":"en"}],"creatorNames":[{"creatorName":"桑名, 俊一","creatorNameLang":"ja"},{"creatorName":"KUWANA, Shunichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"クワナ","familyNameLang":"ja"}],"givenNames":[{"givenName":"シュンイチ","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"490","nameIdentifierScheme":"WEKO"}]},{"creatorAlternatives":[{"creatorAlternative":"HOSOKAWA, Yuki","creatorAlternativeLang":"en"}],"creatorNames":[{"creatorName":"細川, 幸希","creatorNameLang":"ja"},{"creatorName":"HOSOKAWA, Yuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"ホソカワ","familyNameLang":"ja"}],"givenNames":[{"givenName":"ユキ","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"491","nameIdentifierScheme":"WEKO"}]},{"creatorAlternatives":[{"creatorAlternative":"KAKU, Yuki","creatorAlternativeLang":"en"}],"creatorNames":[{"creatorName":"角, 友起","creatorNameLang":"ja"},{"creatorName":"KAKU, Yuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"カク","familyNameLang":"ja"}],"givenNames":[{"givenName":"ユキ","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"492","nameIdentifierScheme":"WEKO"}]},{"creatorAlternatives":[{"creatorAlternative":"SAITO, Kiichiro","creatorAlternativeLang":"en"}],"creatorNames":[{"creatorName":"齋藤, 基一郎","creatorNameLang":"ja"},{"creatorName":"SAITO, Kiichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"サイトウ","familyNameLang":"ja"}],"givenNames":[{"givenName":"キイチロウ","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"493","nameIdentifierScheme":"WEKO"}]},{"creatorAlternatives":[{"creatorAlternative":"KOIKE, Kazuko","creatorAlternativeLang":"en"}],"creatorNames":[{"creatorName":"小池, 和子","creatorNameLang":"ja"},{"creatorName":"KOIKE, Kazuko","creatorNameLang":"en"}],"familyNames":[{"familyName":"コイケ","familyNameLang":"ja"}],"givenNames":[{"givenName":"カズコ","givenNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"494","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-26"}],"displaytype":"detail","filename":"KJ00008523560.pdf","filesize":[{"value":"738.5 kB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"KJ00008523560.pdf","url":"https://uekusa.repo.nii.ac.jp/record/290/files/KJ00008523560.pdf"},"version_id":"c8e25b2b-f09a-4d60-b56e-080604f284ed"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"三叉神経","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"三叉神経脊髄路尾側亜核","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"GABA性ニューロン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"脳幹スライス標本","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ノックインマ ウス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Trigeminal nerve","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Trigeminal subnucleus caudalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GABAergic neuron","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Whole cell patch clamp","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transgenic mouse","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"三叉神経脊髄路亜核内神経網におけるシナプス結合の解析 : GAD67-GFPノックイン新生マウスを用いた研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"三叉神経脊髄路亜核内神経網におけるシナプス結合の解析 : GAD67-GFPノックイン新生マウスを用いた研究","subitem_title_language":"ja"},{"subitem_title":"Analysis of neural connections in the trigeminal spinal tract subnucleus caudalis of GAD67-GFP knock-in neonatal mice","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["1","63"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-05-26"},"publish_date":"2017-05-26","publish_status":"0","recid":"290","relation_version_is_last":true,"title":["三叉神経脊髄路亜核内神経網におけるシナプス結合の解析 : GAD67-GFPノックイン新生マウスを用いた研究"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-11-20T08:12:35.867364+00:00"}